Surface remodeling vs. whole-cell hemolysis of reticulocytes produced with erythroid stimulation or iron deficiency anemia.

lamine (PE) and red cell 14C, reflecting cytoplasmic hemoglobin, were measured sequentially in rats given transfusions of doubly-labeled reticulocytes. With reticulocytes from normal rats there was a small decline in the levels of both the membrane and the cytoplasmic labels; the changes were almost arellel, although loss of membrane PE2P exceeded that of ‘4C to a small extent. By contrast, with “stress reticulocytes” from bled donors, there was a markedly disproportionate loss of the membrane label; this asymmetrical loss of membrane material was diminished when recipients had been splenectomized. With transfusions of doubly-labeled reticulocytes from rats with severe iron deficiency anemia, there was a marked loss of both membrane PE-32P and red cell ‘4C which was only moderately asymmetrical. The asymmetrical loss of the membrane label found with stress reticulocytes supports the conclusion that these cells undergo a process of surface remodeling during their maturation in the peripheral blood. The spleen is partly responsible for this process. Normal reticulocytes also appear to undergo a minor degree of remodeling. On the other hand, the almost symmetrical loss of membrane and cytoplasmic label observed with reticulocytes from iron deficient rats indicates that many of the cells in this model of ineffective erythropoiesis are hemolyzed in their entirety. These experiments demonstrate that stress reticulocytes differ under different conditions and may lose cellular material by two, possibly interrelated, mechanisms: surface remodeling or whole-cell hemolysis.